Date of Defense
29-5-2025 2:00 PM
Location
F1-0046
Document Type
Thesis Defense
Degree Name
Master of Science in Food Science
College
CVAM
Department
Food Science
First Advisor
Dr. Akmal Nazir
Keywords
Double Emulsion, W/O/W, Encapsulation, Lactobacillus reuteri, Probiotic
Abstract
Probiotics, known for its wide range of health benefits, have been gaining recognition on its effect on the gut-brain axis. This axis, operated via various pathways, can be modulated by the type and functions of probiotics found in the gut microflora. Lactobacillus are probiotics commonly found residing in the gut and have been extensively used in food for its health benefits. However, probiotics face several stress factors before reaching the gut which hinders it from being functional. The main objective of this thesis is to construct a stable double-emulsion-based delivery system for the probiotic, which will act as a barrier against the harsh conditions of the digestive system. Lactobacillus reuteri DSM 17938 was loaded in a water-in-oil-in-water Pickering double emulsion system, using sunflower seed oil and pistachio oil. The emulsion particle size, stability during storage, encapsulation efficiency, oxidative stability, and the viability during storage and after simulated gastrointestinal digestion were examined. The results showed significant stability of emulsions during storage with no phase separation. Both oils presented high oxidative stability, with sunflower being more stable. The encapsulation efficiency of both oils was similar, 78.4% for pistachio oil emulsion and 77.6% for sunflower seed oil emulsion. Furthermore, L. reuteri encapsulated in pistachio oil emulsions proved higher viability throughout the study. Both double emulsions were able to successfully protect the probiotic during simulated gastrointestinal digestion and the variability was maintained between the desired concentration, 106 – 107 CFU/g, where sunflower seed oil emulsions achieved 6.89 CFU/mL while pistachio oil emulsions achieved 6.49 CFU/mL by the end of the digestion. Overall, double emulsions were proven to be an excellent method of probiotic delivery system. With some enhancement in the method of construction, as additional source of nutrient for the probiotic, the viability of probiotic-loaded double emulsions is expected to increase.
Included in
ENCAPSULATION OF LACTOBACILLUS REUTERI DSM 17938 IN W/O/W EMULSION: INFLUENCE OF OIL TYPE ON VIABILITY AND OXIDATIVE STABILITY
F1-0046
Probiotics, known for its wide range of health benefits, have been gaining recognition on its effect on the gut-brain axis. This axis, operated via various pathways, can be modulated by the type and functions of probiotics found in the gut microflora. Lactobacillus are probiotics commonly found residing in the gut and have been extensively used in food for its health benefits. However, probiotics face several stress factors before reaching the gut which hinders it from being functional. The main objective of this thesis is to construct a stable double-emulsion-based delivery system for the probiotic, which will act as a barrier against the harsh conditions of the digestive system. Lactobacillus reuteri DSM 17938 was loaded in a water-in-oil-in-water Pickering double emulsion system, using sunflower seed oil and pistachio oil. The emulsion particle size, stability during storage, encapsulation efficiency, oxidative stability, and the viability during storage and after simulated gastrointestinal digestion were examined. The results showed significant stability of emulsions during storage with no phase separation. Both oils presented high oxidative stability, with sunflower being more stable. The encapsulation efficiency of both oils was similar, 78.4% for pistachio oil emulsion and 77.6% for sunflower seed oil emulsion. Furthermore, L. reuteri encapsulated in pistachio oil emulsions proved higher viability throughout the study. Both double emulsions were able to successfully protect the probiotic during simulated gastrointestinal digestion and the variability was maintained between the desired concentration, 106 – 107 CFU/g, where sunflower seed oil emulsions achieved 6.89 CFU/mL while pistachio oil emulsions achieved 6.49 CFU/mL by the end of the digestion. Overall, double emulsions were proven to be an excellent method of probiotic delivery system. With some enhancement in the method of construction, as additional source of nutrient for the probiotic, the viability of probiotic-loaded double emulsions is expected to increase.