Date of Award

11-2015

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Dr. Rabah Iratni

Second Advisor

Dr.Synan AbuQamar

Third Advisor

Dr. Adel Sahaban Sadeq

Abstract

In the present project, we extend the previous studies of Origanum majorana by evaluating the anti-cancer potential of this plant extract on proliferation of colon cancer. Toward this, we first evaluated the effect of the plant extract on the proliferation and DNA damage, in vitro, of the human colon cancer HT-29 cell line. We also determine the mechanism by which Origanum majorana induced cell death by this extract using biochemical approach. Our results revealed that O. majorana inhibited the growth of HT-29 cells by effecting the cells viability. Further work showed the ability of the extract to cause DNA damage, cell cycle arrest and apoptotic death through activation of caspase 8 and 3 pathways. In conclusion our data recommend Origanum majorana is an effective inhibitor for colon cancer progression and more investigation has to explore the Origanum majorana extract in vivo.

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