Date of Award
Master of Science (MS)
Raafat Abd El-Gharib El-Awady
Unsolved riddle of cancer remains a major global health concern particularly in hepatocellular carcinoma (HCC) which is among the leading causes of cancer related death worldwide. Saffron (Crocus sativus L.) and its bioactive phytochemicals possess promising anti-cancer activities. One of these phytochemicals, safranal, has demonstrated potent anti-cancer activities against different types of cancers. Yet, its association with liver cancer remains under documented. This study investigated HCC response to safranal’s treatment via transcriptomic approach. Safranal was found to be involved in mitochondrial dysfunction, induced oxidative stress and increased reactive oxygen species (ROS) production leading to cell death. Interestingly, safranal has seemingly interfered with mitogen-activated protein kinase (MAPK) signaling pathway that is responsible for cell growth, proliferation, differentiation and survival. Through ingenuity pathway analysis (IPA), 45 genes were associated with HCC inhibition. To further pinpoint specific genes, IPA re-analysis revealed 8 genes to be associated with decrease in tumor’s incidence and angiogenesis. Taken together, this study unravel safranal’s novel potential as a natural therapeutic agent and a much needed adjuvant against HCC.
Ahmed Baig, Badriya Mahmood, "Transcriptomic Analysis on Hepatocellular Carcinoma Model in Response to Safranal Treatment" (2018). Biology Theses. 14.