Date of Award
Master of Science (MS)
Shyam S. Kurup
Genomic assets from the plants innate to abiotic stress environments have the prospective to be latent in generating stress tolerant plants. Late embryogenesis abundant (LEA) proteins are decisive phytomolecules, which accumulates mainly in the late stages of seed development and in the vegetative tissues during exogenous stresses as a reaction to stabilize the damaged macromolecules. In spite of substantial research, their mechanism of action to initiate plant tolerance against abiotic stresses remains enigmatic. Here, the study is a preliminary investigation on LEA2 genes from the P. dactylifera cv. khalas, a relatively tolerant plant of arid regions. In this aspect, the current study was undertaken to clone five LEA2 genes from date palm (DpLEA2) cv. khalas and characterize their functionality towards heat and salt stress tolerance using yeast knockout mutants. Through the utilization of whole transcriptome sequencing of date palm, it was found that the DpLEA2 genes were highly expressed in roots when the seedlings were challenged with salt stress. A total of five DpLEA2 genes were isolated, and analysed using bioinformatic tools, which displayed high sequence similarity and possession of certain specific conserved motifs. To evaluate for their possible functions in stress tolerance, three DpLEA2 genes from the five isolated genes were expressed in Saccharomyces cerevisiae (AXT3K, W303 and CDC25 strains), a genetically tractable eukaryotic facsimile organism. Heat stress experiments showed that all the three DpLEA2 genes significantly enhanced the transformed CDC25 yeast strain survival and viability, with an effective protection to the yeast cells, while for the salt stress experiments only DpLEA2.4 gene provided protection to transformed W303 yeast cells, with significantly enhanced tolerance observation. In grasp of, the results set forth that DpLEA2 genes may have a remarkable part in amending the tolerance and survival ability of plants or organisms under heat and salt stresses. This could account them as promising candidate genes for heat and salt stress tolerance transgenic plant researches and as a forthcoming functional molecular marker for marker-assisted selection in P. dactylifera genomic breeding programs for heat and salt stress tolerance.
Abdul Aziz, Mughair, "CLONING AND SEQUENCING OF FIVE LEA2 GENES FROM DATE PALM CV. KHALAS AND FUNCTIONAL CHARACTERIZATION TO HEAT AND SALT STRESS TOLERANCE USING YEAST KNOCKOUT MUTANTS" (2020). Agribusiness Theses. 2.