Date of Award
Master of Science (MS)
Shyam S. Kurup
Adel D. Al-qurashi
The sustainable green synthesis process for the development of environment-friendly, toxicant-free and biocompatible nanoparticles establishes important aspects in nanotechnology research. Even though; nanoparticles can be produced by either conventional physical and chemical or green synthesis, synthesizing nanoparticles using plant extracts as reducing and capping agents is acceptable. The benefits of plant extract mediated synthesis of silver nanoparticles comprise economic viability, quick synthesis, toxicity free, cost-effectiveness and large scale synthesis. In that way, the aqueous extract of Moringa peregrina leaves was used to synthesize silver nanoparticles. The silver nanoparticles were characterized by various spectral studies including FT-IR, SEM, HR-TEM and XRD. The silver nanoparticles were studied for antioxidant activity viz. DPPH, ABTS, Hydroxyl radical scavenging, Superoxide radicals scavenging, Nitric oxide scavenging potential and reducing power with varied concentrations. The silver nanoparticles showed good antioxidant activity compared with Gallic acid. The anticancer potential of the nanoparticle was studied against MCF-7 (Breast cancer cells) and Caco-2 (colorectal cancer cells). The results revealed that the nanoparticles showed good toxicity of the studied cancer cell lines with the IC50 values of 41.59 (Caco-2) and 26.93 (MCF-7) μg/ml. The results were compared with the standard Doxorubicin. In conclusion, the biosynthesized nanoparticles using M. peregrina as a reducing agent showed good antioxidant and anticancer potential on human cancer cells and it can be used in biological applications. However, more studies on surface modification and of AgNPs during biosynthesis and signaling mechanism behind the action are warranted for a better understanding of its bioactivity. Moreover, the cytotoxicity against normal cells also needs to be analyzed.
Al Baloushi, Khaled Saeed Yousef, "GREEN SYNTHESIS, CHARACTERIZATION, ANTIOXIDANT AND ANTICANCER ACTIVITIES OF SILVER NANOPARTICLE USING MORINGA PEREGRINA LEAF EXTRACT" (2020). Agribusiness Theses. 1.